Generator

Part:BBa_K1404002:Design

Designed by: Alexandre Duprey   Group: iGEM14_INSA-LYON   (2014-09-23)

Ptac-NiCoTB, improves nickel and cobalt internalization


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 857
    Illegal AgeI site found at 1056
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This parts associates the strong constitutive promoter Ptac (as found in the plasmid pKK233-2, Clontech), a strong RBS desgined by team Paris Bettencourt 2009, a nickel and cobalt transporter originating from the locus Saro_0344 of Novosphingobium aromaticivorans DSM 12444 which was codon-optimised for expression in E. coli and the bidirectional terminator B0014 , to avoid cross-talk between this part and K540000 in the composite part K1404001

Source

The promoter and the rest of this part were synthesized separately and then assembled by standard assembly.

References

[1] Duprey A, Chansavang V, Fremion F, Gonthier C, Louis Y, Lejeune P, Springer F, Desjardin V, Rodrigue A, Dorel C: “NiCo Buster”: engineering E. coli for fast and efficient capture of cobalt and nickel. J Biol Eng 2014, 8:19.